About BioTechniques
WEBYear of la unch: 1983 ISSN (print): 0736-6205 | ISSN (online): 1940-9818 Frequency: 12 issues per year – Impact Factor: 2.746 (2021). Established in 1983, BioTechniques was founded on two ground-breaking and inspired principles: First, and most critically, seeing the need for a journal to properly communicate methods, techniques and new instrumentation to those researchers working in the lab ...
An introduction to Droplet Digital PCR - BioTechniques
WEBOct 13, 2022 · This video serves as an introduction to Droplet Digital TM PCR, a highly sensitive technology for quantifying target RNA or DNA. Learn about how it works, what it’s used for and how it can offer streamlined approaches to quantification and amplification of DNA or RNA in rare and precious samples. Animation by Kristian Baycroft-Willmott.
Western Blotting Summary - BioTechniques
WEBWestern blotting (or immunoblotting) is a well-established biochemical technique that detects specific proteins in complex samples and has applications from basic research to the diagnosis of infectious diseases. Western blotting can be used to determine quantity, molecular weight and post-translational modifications of proteins and can ...
Next-Generation Sequencing Summary - BioTechniques
WEBNext-generation sequencing (often noted as NGS), describes the high-throughput technologies that can now determine the sequence of a given nucleic acid strand, such as DNA or RNA, in a matter of hours or days. These technologies have revolutionized genomics research as we know it. A number of different …
MinION Mk1C: All-in-one, portable DNA and RNA sequencing
WEBMar 11, 2022 · MinION nanopore sequencers are the only portable, real-time devices for DNA and RNA sequencing, giving complete control and creativity over when, where and how often you sequence, regardless of application. The MinION Mk1C has been created with a high-resolution touchscreen, for simple device control and visualisation of results.
Aims and scope - BioTechniques
WEBBioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science ...
Protecting the powerhouse of the cell - BioTechniques
WEBJan 11, 2019 · The mitochondria, often labeled the powerhouse of the cell, are the organelle responsible for energy production within the cell. Playing an important role in cellular respiration, the mitochondria are the main location for ATP production. They also make proteins essential for DNA metabolism such as the iron-sulfur family of proteins.
What is Western Blotting? The Story of the Western Blot
WEBJul 18, 2018 · The technique involves the identification and characterization of DNA-binding proteins using DNA probes (Southern) and protein blotting (western) and was first described in 1980. This method was published before the western blot. Yet, it still alludes to it in its name, reaffirming the popularity of the western blot.
What is DNA Sequencing? - BioTechniques
WEBSep 16, 2019 · DNA sequencing refers to the process of identifying the order of bases in a strand of DNA. DNA is comprised of nucleotides, with each nucleotide consisting of a sugar, a phosphate group and a base. Within double stranded DNA (dsDNA), bases from single stranded DNA (ssDNA) will form pairs with the bases on the other ssDNA.
How Early Life Thrived Without Oxygen - BioTechniques
WEBJan 11, 2017 · According to Planavsky, oxygen saturated the surface of oceans and the atmosphere, but these sulfur-oxidizing bacteria thrived best at the interface between oxic and oxygen-poor waters. The evolution of the environment changed the course of evolution for most life on Earth. “A lot of people don’t think about how the Earth has changed so ...