We reviewed the best free Adobe Illustrator alternatives for managing graphics files without a Creative Cloud subscription. We've rounded-up our top-rated choices for when you want to make the ...

An overlooked jewel hugging its very own lake—Lake Lugano—the city has long been a haven for writers and artists drawn to its palm tree-lined beauty, Alpine foothills, warm summer weather ...

To expand the size of inserted DNA fragments in the shotgun metagenomic library, a topoisomerase (TOPO)-adapted vector was employed and the library construction protocol was fine-tuned. As a reporter ...

The PCR products were subcloned into the pCR-II TOPO vector (TOPO-TA kit; 45-0640, Invitrogen, Carlsbad, CA, USA) and confirmed by restriction digests and DNA sequencing. For riboprobe synthesis, the ...