We describe a general strategy forthe genetic mapping in parallel of multiple restriction fragment length polymorphism (RFLP) loci. This approach allows the systematic identification for cloning of physical …

The quantity of lin-4 miRNA, relative to U6 as an internal reference gene, was calculated using the formula 2-DCT, where DCT= (CT lin-4 – CT U6). The lin-4 level was then normalized to its level at 0 …

Transferring the lin-14 3'UTR into a reporter gene resulted in post-transcriptional regulation of the reporter gene that was similar to lin-14, demonstrating that the heterologous 3'UTR was sufficient to …

Although lin-3 affects other developmental processes, for example a putative null mutation in lin-3 results in lethality early in postembryonic development6, only the role of lin-3 in vulval ...

Transferring the lin-14 3'UTR into a reporter gene resulted in post-transcriptional regulation of the reporter gene that was similar to lin-14, demonstrating that the heterologous 3'UTR was sufficient to …