SOLVED:How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?
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SOLVED:To gauge the fidelity of DNA synthesis, Arthur Kornberg and colleagues devised a technique called nearest-neighbor analy- sis, which determines the frequency with which any two bases occur adjacent to each other along the polynucleotide chain (J. Biol. Chem. 236: 864-875 ). This test relies on the enzyme spleen phosphodiesterase (see the previous problem). As we saw in Figure 11-8, DNA is synthesized by polymerization of 5^' -nucleotides -that is, each nucleotide is added with the phos- p
SOLVED:To gauge the fidelity of DNA synthesis, Arthur Kornberg a…
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